- [2018-03-24] Report of clinical trials of high frequency modulated torsion field application to female post-reproductive period organisms for the purpose of its geroprotective and bioadaptive effect evaluation
REPORT
of clinical trials of high frequency modulated torsion field application to female
post-reproductive period organisms for the purpose of its geroprotective and
bioadaptive effect evaluation
23.09.2017
Research group, consisting of: S. Winkler, V. Nesterov, A. Schmidt, S.
Oliveri, T. Fridman, A. Topaller (leader of the group – Professor S. Winkler), had
been performing a research of high frequency modulated right-hand polarized
torsion field effect for the purpose of evaluation of its geroprotective and bioadaptive
effect to women’s organisms during post-menopause period, since 6.02.2016 till
14.04.2017.
To perform this study Institute of Practical Psychophysics together with
“Clinic Tech Incorporated” have developed an experimental device “Metatron Z-
100” (TU-9442-004-11907401-2005), which allows bringing effect of highfrequency
(with 100 GHz field generation rate) torsion field with low-frequency
(250 Hz) broad-impulse signal modulation, with chopping step within 1.8 – 8.2 Hz,
tuning spacing of signal chopping frequency – 0.02 Hz and pulse ratio from 0.1% to
99%.
Device had an additional feature of influencing organisms with separate righthand
and left-hand polarized field in accordance with “protector-destructor”
principle.
We carried out a study of torsion field effect on 12 women volunteers, forming
a main group, aged 51 – 58. Average age of women in this group was 54.8±3.7.
Control group consisted of six women aged 53-57, whose average age was
55.1±2.8.
All women signed an informed consent for participation in clinical trials. No
complications or side effects were found during studies.
All women of both a main and a control group were in their menopause period,
all of them had no menstruations for more than two years. In a main group, 10
women were in a menopause period for 2-5 years and two women were in a
menopause period more than 5 years. In a control group, all women were in a
menopause period for 2-5 years.
Throughout the experiment (12 months) organisms of women from a main
group were subjected to effect of right-hand polarized torsion field, once in a week,
with 10 minutes exposition. No effects were administered to women of a control
group.
Statistical processing of acquired data was carried out according to standard
methods; reliability of differences was evaluated by Student’s criterion.
Study results
1. Evaluation of women’s objective condition
In 5-8 months after beginning of torsion field exposure, the first signs of
rejuvenation appeared in women of the first group. Subjectively all women noted
improved well-being, memory, absence of depressions, decreasing of nervousness,
improved sleep. In all women, without exception, we registered increased muscle
tone, improved skin elasticity, decreasing of sagginess and smoothing of wrinkles.
Majority of women noted enlargement of mammary glands. Nine women also noted
improvement of eyesight. Seven women noted complete disappearance of pain
sensations along spine and in lumbar area during walking and standing, joints pain,
which were registered in them previously. In four women hair loss process, which
was noted before, stopped.
2. General blood characteristics.
Analysis of peripheral blood characteristics of women from both main and
control groups proved that both groups had similar tendencies of changes within
whole period of observation. So, with increasing of hemoglobin level, distinctions
between groups were not manifested.
The same tendency was observed in relation to increasing of red blood cells
number. However, the increase (∆) of these values in a main group was 2.3 times
higher than in a control group. Color index decreased equally in both groups.
Absolute content of leukocytes in peripheral blood decreased as well. At the same
time difference between initial and final values of Le content in a main group was
reliably higher (Р >0.5) than in a control group. Taking into consideration that initial
values in both groups were at upper limits of normal values, decreasing of Le content
in a main group of woman during experiment, evidenced the optimization of
leukocytes quantitative composition.
3. Structure of general adaptive response.
Blood leukocytes qualitative composition, expressed through blood formula
by Shilling, is an identification criterion of general non-specific adaptive response
of an organism.
Evaluation of adaptive response structure (training – T; quiet activation – QA;
strong activation – SA; stress – S) in women from a main and a control groups in
initial condition revealed that all studied women were in chronic stress condition.
At the same time, low level of lymphocytes – the main signal criterion of
adaptive response, forming on average 45% in a main group, was combined with
signs of response intensity (monocytosis higher than 10%, eosinophiles increase up
to 5-7%), which evidenced development of stress at very low levels of reactivity.
Structure of initial adaptive response of women from a control group differed
in identification of not only stress response, but also in training response related to
anti-stress physiological type of responses.
Correlation of various by character types of response may be expressed
through coefficient C(QA/S) which makes possible to find out anti-stress potential
in groups structure. In a main group C(QA/S) was equal to 0, in a control group it
was 1.6, which evidenced better initial position of women in a control group.
Besides, according to degree of intensity these adaptive responses could be related
to low reactivity levels, at the same time in a main group the most negative
(energetically and functionally) very low levels of reactivity prevailed. After
exposure to torsion field, identification of adaptive response revealed withdrawal
from deep stress response and development of training, quiet and strong activation
responses in majority of women. At the same time reactive levels significantly
increased (i.e. shift from very low to average and high reactivity levels was
observed). Even in the single case of stress retaining, background values of
lymphocytes content increased by 15.8%, decreasing of monocytic intensity by
3.6%, which evidenced shift from very low to high reactivity levels. It is important
that C(QA/S) from 0 increased to 3.8.
In a control group of women, change of adaptive conditions was also
observed: shift from training response into stress or strong active and shift from
stress into quiet and strong activation (3 women). Together with improvement of
general group structure of responses, which appeared in increasing of C(QA/S) in
3.2 times, retaining of responses intensity signs characterizing low levels of
reactivity was marked.
Thus, comparison of acquired data makes possible to single out the following
peculiarities of general non-specific adaptive responses development in women at
exposure to high-frequency torsion field.
1) withdrawal from deep stress condition of very low reactivity levels.
2) forming of anti-stress responses of average and high reactivity levels.
The following signs were typical for a control group: shifting of adaptive
responses to more active forms, while having the same low levels of reactivity.
4. Endogenous intoxication values.
It is known that endogenous intoxication, being a non-specific syndrome, is
typical for various pathologic conditions, including those appeared at ageing of an
organism. Evaluation and correction of endogenous intoxication syndrome became
very important for verification of rejuvenation processes.
We have studied cell tests of reactivity and intoxication, calculated according
to blood leukogram.
Index of leukocytes shift:
ILS = (e+b+n)*(m+lph) = 1.94±0.42 (norm)
Lymphocytic index of intoxication:
LI = lph/n = 0.55±0.04 (norm)
Leukocytic index of intoxication according to Kalf-Kalif:
LII = (4mc + 3y + 2st + s)*(pl + 1)*(m + lph)*(e +1) = 0.3/1.6 (norm)
Where e - eosinophiles, b – basophiles, n – neutrophil (st -stab, s -segmented),
m – monocytes, lph - lymphocytes, mc - myelocytes, pl - plasmacytes, y - young.
Analysis of women’s reactivity and intoxication test results in observed
groups showed the following. In initial condition, total amount of normative
intoxication tests in a control group was 1.5 times higher than in a main group. At
the same time group difference in frequency of normative tests revealing gradually
increased starting from ILS, then LI and finally LII, which represents the most
precise picture of toxic-dystrophic condition of blood cell systems, and indirect
value of detoxicating functions of an organism. Therefore, observed level of
correspondence in intoxication indices norm in a control group evidenced
satisfactory condition of protective functions and their weakening in women from a
main group.
After exposure to torsion field the situation changed due to redistribution of
normative tests: in women from main group, in all cases normal values of
intoxication indices were completely restored: ILS, LI, LII.
In a control group, only one woman retained ILS and LII test normal values,
and in one case LII index within normal values. In absolute majority of cases,
standards of ILS, LI, LII were breached and their level was decreased by 5 times in
comparison with background values. Thus, if we only consider total amount of
standard intoxication tests in a main group without discussing of their intra-group
distribution, we may note reliable difference with a control group (Р<0,001), where
quantity of standard values of ILS, LI and LII indices decreased by 3.2 times. It
evidenced that without effect of torsion field, natural course of protectivedetoxicating
processes in organism prevailed, however application of highfrequency
torsion field promoted their activization.
5. Torsion field effect to a functional potential of blood neutrophilic
granulocytes.
Protective function of neutrophilic granulocytes, as cell factors of non-specific
resistance, is widely known (G.E.Brill et al., 1995). Cationic proteins, defined by
lysosomal-cationic test (LCT), are the main factor, ensuring functional potential of
neutrophils.
In blood preparations, taken from women from main and control groups,
colored by green azure A, we defined mean cytochemical coefficient (MCC) in
mature neutrophiles. In each blood preparation, we processed at least 100 cells and
calculated MCC after Astaldi and Verg. Initial values of MCC in both groups are
decreased to the same level, which evidences decreasing of cells percentage
containing cationic proteins granules. After completion of the experiment in a
control group MCC values remained at the initial low level, but in women from a
main group, which were subjected to high-frequency torsion field effect, MCC
values proved reliable increase of cells with high amount of cationic proteins content
(Р<0,001).
Observed increase of MCC values by 6.2 times after exposure to torsion field,
obviously showed improving of detoxicating resistance as one of the mechanisms of
organism resistance improving.
In its turn mobilization of organism’s natural resistance cells, manifested in
increasing of neutrophiles number with high LCT values after exposure to torsion
field, was confirmed and coordinated with reactivity and intoxication cells tests, and
increase of its standard values was observed in a main group.
Besides, high-amplitude dynamics of lysosomal-cationic test correlated with
significant increasing of reactivity levels by parameters of general adaptation
responses, at the same time in a control group reactivity levels remained lower,
despite anti-stress character of adaptation response.
In other words, exposure of ageing women to high-frequency torsion field,
promoted synchronization of processes, happening at a cell level and integral
response of an organism, including multi-system mechanisms.
6. Restoration of reproductive function in women after exposure to
torsion field.
All women in both main and control group were in their menopause period,
end climacteric stage, when menstruation completely stopped more than two years
ago.
In 6-9 months after beginning of the experiment, in six women, subjected to
torsion field effect, menstruations reappeared and restoration of duration and
sequence of all stages of sexual cycle (follicular, ovulatory and lutein (secretory))
was registered.
In a control group, no significant changes of declining reproductive function
in women were registered.
Changing of a hormonal background allowed ascertaining the following:
Before exposure to torsion field effect, in both groups (main and control) we
noted physical decrease of estradiol level (21.5±1.09 pmole/l), increased
testosterone content (2.5±0.5 mmole/l), increased content of follicle-stimulating
hormone (152.75±12 mmЕ/ml) – typical for postclimacteric period.
Over the course of female organisms’ exposure to torsion field, estradiol level
in a main group increased by 4.8 and 9.2 times in 6 and 12 months correspondingly
in comparison with the initial levels. Estradiol level in a control group decreased by
2.3 times within the period of study.
Level of follicle-stimulating hormone in women of a main group decreased
by 6.2 times in comparison with the initial level, testosterone content decreased by
5.2 times on average. In a control group, these indices have not changed reliably
during the study.
Conclusion
By summarizing of acquired data, we may ascertain marked bioadaptive and
geroprotective effect of high-frequency modulated right-hand polarized torsion
field. Non-specific mechanisms of its effect to female organisms were registered
thanks to study of interrelated blood characteristics, including increasing of red
blood cells content and decreasing (normalization) of leukocytes general amount,
increasing of immunocompetent cells content. Using multi-component cell contents
of blood and with help of estimated reactivity and intoxication indices we managed
to define reconstruction of protective-detoxicating processes standard values in
women after exposure to torsion field.
Surely, endocrine regulation is the oldest form of control, and it is subjected
to significant involution during ageing. Due to this fact, complete restoration of
hypothalamic-gonadotropic regulation and hormonal status in ageing women after
exposure to torsion field, is the most significant result. Therefore, we achieved not
only metabolic and visible rejuvenation of women, but also restored childbearing
function.
______________________ S. Winkler,
______________________ V. Nesterov
______________________ A. Schmidt
______________________ S. Oliveri
______________________ T. Fridman
______________________ A. Topaller
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